Creating Glycerol Stocks in FluidX Tubes

Description

This protocol creates duplicate glycerol stocks from colony suspensions. Two racks of Fluid X tubes are prepared with glycerol prior to sample transfer from a 96 deep well plate.

Labware and Liquids

Right mount: An 8-channel p300 pipette (GEN2)
Slots 1 and 2: Racks with 96 FluidX tubes
Slot 4: Reservoir with relevant amount of glycerol
Slot 5: 96 Deep well plate containing colony suspensions
Slots 7 and 10: Tip racks with 300ul tips

Execution

Set up the deck as described above.
Run the protocol.

Script

Creating Glycerol Stocks in FluidX Tubes

Tips and best practices

Carry out a test run of the protocol using the labware and water to ensure that the positioning of labware and tips is suitable. Re-calibration of the deck or labware may be required.