PCR Purification Using Magnetic Beads

Description

This protocol purifies a rack of 96 PCR samples utilising magnetic beads and the magnetic module.

Labware and Liquids

Left mount: An 8-channel p20 pipette (GEN2)
Right mount: An 8-channel p300 pipette (GEN2)
Slot 1: Reservoir to collect liquid waste
Slot 4: Magnetic module (GEN2) with 96 well PCR plate containing 20µl of PCR product
Slot 7: Twelve channel reservoir containing beads, ethanol wash and elution buffer
Slots 2, 3, 5, 6, 8, and 9: 300µl Tip Racks
Slot 11: 20µl Tip Rack

Execution

Set up the deck as described above.
Run the protocol.
The protocol contains pauses and manual steps to empty the waste box and check the beads.

Script

PCR Purification Using Magnetic Beads

Tips and best practices

Carry out a test run of the protocol using the labware and water to ensure that the positioning of labware and tips is suitable. Re-calibration of the deck or labware may be required.

Ensure the beads are fully dry prior to elution to prevent any ethanol carry-over into the final elution.