Transformation Spreading to 6 Well Plates

Description

This set of protocols transfers samples from a 96 well plate to 6-well plates containing solid media.

There are four separate protocols which carry out the transfer of 24 samples to four 6-well plates, resulting in 96 samples being transferred to sixteen 6-well plates.

Labware and Liquids

Left mount: An 8-channel p300 pipette (GEN2)
Slot 1, 4, 7 and 10: 6-well culture plates containing solid media
Slot 3: 300µl Tip Rack
Slot 6: 96 Well PCR Plate containing the samples

Execution

Select the correct protocol for the set of samples requiring transfer.
Set up the deck as described above and as in the initial protocol pause step.
Run the protocol.

Script

Transformation Spreading to 6 Well Plates - Script 1

Transformation Spreading to 6 Well Plates - Script 2

Transformation Spreading to 6 Well Plates - Script 3

Transformation Spreading to 6 Well Plates - Script 4

Tips and best practices

Carry out a test run of the protocol using the labware and water to ensure that the positioning of labware and tips is suitable. Re-calibration of the deck or labware may be required.

Label the 6-well plates clearly to ensure the traceability of the samples from the PCR plate to the 6-well plates.

Following completion of the protocol(s) gently roll the plates to ensure that the sample is spread across the solid media.