Bacterial Transformation Protocol

Description

This set of protocols carries out bacterial transformation of 384 low volume samples.

There are four separate protocols which carry out the transformation of 96 samples from a specific quarter of the 384 well plate.

Labware and Liquids

Right mount: An 8-channel p20 pipette (GEN2)
Slot 1: 96 well PCR plate containing out-growth media
Slots 3, 6, 9: 20µl Tip Racks
Slot 4: Temperature module (GEN2) with 384 PCR plate containing samples for transformation
Slot 5: 12-channel reservoir containing out-growth media
Slot 7: Aluminium block containing a strip of PCR tubes with competent cells in column 1

Execution

Select the correct protocol for the set of samples requiring transformation.
Set up the deck as described above and as in the initial protocol pause step.
Run the protocol.
There are various pause steps and manual steps requiring immediate interaction with the instrument.

Script

Bacterial Transformation Protocol - Script 1

Bacterial Transformation Protocol - Script 2

Bacterial Transformation Protocol - Script 3

Bacterial Transformation Protocol - Script 4

Tips and best practices

Carry out a test run of the protocol using the labware and water to ensure that the positioning of labware and tips is suitable. Re-calibration of the deck or labware may be required.

Ensure the aluminium block has been placed in the freezer prior to being placed on the deck. This will keep the competent cells cold and prevent reduced efficiency of the process.