PCR Plate Preparation

Description

This protocol transfers reagent and samples to a 384 well PCR plate ready for PCR amplification.

Labware and Liquids

Left mount: An 8-channel p20 pipette (GEN2)
Right mount: An single-channel p20 pipette (GEN2)
Slots 1, 3, 4, and 6: 96 well rack containing PCR strips in every other column. The PCR strips contain the samples for PCR.
Slot 2: Eppendorf tube rack containing 1 x 2ml tube containing reagents.
Slot 5: 384 well PCR plate
Slots 7, 8, 9: 20µl Tip Racks

Execution

Set up the deck as described above and as in the initial protocol pause step.
Run the protocol.

Script

PCR Plate Preparation

Tips and best practices

Carry out a test run of the protocol using the labware and water to ensure that the positioning of labware and tips is suitable. Re-calibration of the deck or labware may be required.

Ensure that the strip tubes are in the odd columns of each tube rack. If tube racks are unavailable a PCR plate can be used to hold the PCR strips.