Transformation of plasmids in 96-well format

Description

This protocol carries out the transformation of a 96-well plate with chemically competent cells with plasmids in another 96-well plate

For help with how to run Jupyter Notebook protocols, refer to our tutorial

Labware and Liquids

Left mount: An 8-channel p20 pipette
Right mount: An 8-channel p300 pipette
Slot 1: DNA plate (96-well PCR plate)
Slot 3 (don't place the plate here yet): Competent cell plate on temperature module (96-well PCR plate)
Slots 4 and 7: Tipracks for both pipettes
Slot 5: 12-well reservoir for LB

Execution

Set up the deck as described above
Run the cells one by one, paying attention to prompts and instructions

Script

Transformation of plasmids in 96-well format

Tips and best practices

This is a protocol with a manual step. You will have to transfer the competent cells into the robot deck mid-protocol and out to an external thermocycler

It might be usefulk having a bucket of ice to move the competent cell plate to/from the robot deck

Custom labware

enzymax_12_reservoir_20ml